Encapsulation-free controlled release: Electrostatic adsorption eliminates the need for protein encapsulation in PLGA nanoparticles

Encapsulation of therapeutic molecules within polymer particles is a well-established method for achieving controlled release, yet challenges such as low loading, poor encapsulation efficiency, and loss of protein activity limit clinical translation. Despite this, the paradigm for the use of polymer particles in drug delivery has remained essentially unchanged for several decades. By taking advantage of the adsorption of protein therapeutics to poly(lactic-co-glycolic acid) (PLGA) nanoparticles, we demonstrate controlled release without encapsulation. In fact, we obtain identical, burst-free, extended-release profiles for three different protein therapeutics with and without encapsulation in PLGA nanoparticles embedded within a hydrogel. Using both positively and negatively charged proteins, we show that short-range electrostatic interactions between the proteins and the PLGA nanoparticles are the underlying mechanism for controlled release. Moreover, we demonstrate tunable release by modifying nanoparticle concentration, nanoparticle size, or environmental pH. These new insights obviate the need for encapsulation and offer promising, translatable strategies for a more effective...

Toronto sculptor, 59, gets heavy-metal poisoning after spending 15 years with mussel shells

A Toronto artist who has been sculpting with natural materials since 1991 was shocked when she learned that mussel shells she had worked with for 15 years caused her to suffer heavy-metal poisoning.  Gillian Genser, a 59-year-old sculptor based in Toronto, began using blue mussel shells in 2000 while working on the sculpture of Adam, the first man. She had made a statue of Lilith, just before, but using eggshells. ‘The shells came from Atlantic Canada, and I’d buy them in bulk in Chinatown, so that I could sort through the bins and choose shells in the shapes I wanted,’ she wrote in a personal essay for Toronto Life.  Gillian Genser, a 59-year-old sculptor based in Toronto, began using blue mussel shells in 2000 while working on the sculpture of Adam, the first man She said: ’I spent up to 12 hours a day grinding and sanding the shells to fit into the shape of Adam’s body. They beautifully replicated the striations in his muscle fibres. I sifted through thousands of mussels and served them to friends and ­family two or three times a week’ ‘I spent up to 12 hours a day grinding and sanding the shells to fit into the shape of Adam’s body. They beauti...

‘Big Tex’: largest captive alligator in US found after escaping in Imelda flooding | US news

Alligator found near a pond on the Gator Country property. A number of his smaller friends, however, are still missing Residents of Beaumont, Texas, who saw this summer’s alligator horror movie Crawl might have suffered more sleepless nights this week, after the largest gator ever captured in the US was reported missing amid flooding caused by Tropical Storm Imelda. Crawl, which the Guardian said “chomps off exactly the right amount to chew”, deals with the plight of a father and daughter menaced by alligators in a hurricane-flooded home. After Imelda dropped 43in of rain in 72 hours, the Gator Country Adventure Park found itself submerged. When staff checked, Big Al, an 84-year-old 13-footer, was still in contented residence. But Big Tex, nearly 14ft long and weighing 1,000lbs, was gone. “We were unsure he had even escaped,” Jon Warner, alligator program leader for the Texas Parks and Wildlife Department, told the Washington Post. “But when the water level went down, Big Tex wasn’t there.” Gator Country founder Gary Saurage told the Beaumont Enterprise Tex would be found, as after all: “You can’t miss an alligator that big.” And on Friday, once the waters had receded, Big Tex ...

Model-based measurement and inspection software work with CMMs, enabling stampers to compare a first part to

Model-based measurement and inspection software work with CMMs, enabling stampers to compare a first part to the nominal 3D CAD model quickly. Image courtesy of Verisurf Software, Anaheim, Calif. In this era of recalls and defect-caused product lawsuits, automotive, aerospace, medical device, and appliance OEMs are increasingly demanding that their stamping suppliers provide defect-free parts—and sometimes levy penalties if the parts they receive are defective. Sometimes stampers are instructed to deliver their parts directly to their customer’s customers, making “100 percent defect-free” more critical than ever. They are expected to ensure accuracy, consistency, and repeatability. After all, one bad part in a customer’s assembly process can create hundreds of hours of rechecks and additional production. Many stampers contend that they cannot guarantee that defective parts won’t come off the press, but they can guarantee the bad parts won’t go out the door. They check, double-check, and triple-check the stamped parts to screen out the bad ones. How? By running the parts through a series of quality verification devices. Stamping manufacturers may use a physical check fixture, vis...

This surprisingly good $100 portable projector is on sale right now for $69.99

The quality of smartphone screens has improved dramatically over the past few years to the point where most people have a phone with a better display than the one on their TV. That said, they’re still too tiny for multiple people to enjoy at once, and that will never change. If you want a little gadget that will make viewing content on your phone immeasurably better for multiple people, you need the XIAOYA T8 Mini Projector. It’s impressively bright at 3,200 lumens, surprisingly clear in low lighting, and it’s on sale right now for only $69.99 instead of $100! Follow @BGRDeals on Twitter to keep up with the latest and greatest deals from around the web. Quantities may be limited. Prices are subject to change without notice and coupons may expire at any time. Some deals may not be available to all customers. BGR may receive a commission on orders placed through this article. Glasses Free 3d Player, Led Video Wall, 3d Led Video Wall – Shikun,https://www.aerial-display.com/

Dish towels are great. At a pinch, they do everything. Dry a dish. Wipe a counter.

Dish towels are great. At a pinch, they do everything. Dry a dish. Wipe a counter. Get some schmutz off your hand. But all that stuff stays on the towel. So if you can’t keep the dish drying towel separate from the schmutz-getting towel, you’re going to mix them up. And that means, well, schmutz on your dishes. It also means you might need to upgrade your towels and do so with some intention. We put together some of the best-selling and top-rated dish towels you should consider, all of them on Amazon and easy to buy, get shipped, and return (just in case). Utopia Kitchen Towels, 12-Pack, $16 on Amazon: Classics, subtle, and very highly-rated, this towel set’s earned a 4.5-star average rating from more than 1,500 reviewers. You get six white and six black towels, each built with a dobby weave to allow them to dry quickly. Swedish Dishcloth Cellulose Sponge Cloths, 10-Pack, $20 on Amazon:  The cellulose making up these hybrid towels allows them to absorb more than 20 times their initial weight. You can ten of them in five different colors: teal, orange, purple, red, and blue. Soft to the touch but gritty enough to scrub the grime, this is a great option to sort out your daily nee...

Ideal for boundary security, our focus is to offer a selection of systems which Deter, Detect,

Ideal for boundary security, our focus is to offer a selection of systems which Deter, Detect, Delay and Deny unauthorised access Provides ideal solution for a wide variety of high security perimeter and access control applications We offer a variety of solutions in timber and steel for school, playground and play area applications Designed to match our Anti Trap Bow Top Fencing, conforming to all current play area safety guidelines. Security Toppings that increase levels of security against intrusion, damage, acts of vandalism or terrorism Highly effective alternatives to metal fencing when used to provide protection and access control around electrical equipment Constructed from 250mm wire double skinned mesh with clamp bars added and anti tamper security fixings. ’358′ mesh panels are welded on both faces, with reinforced tubular vertical bars, horizontal rails and concertina barbed tape topping. The highest possible security rating for fencing. Can withstand serious attempts at forced entry with battery powered tools. ’358′ Securi-Mesh® clad on both sides with reinforced tubular vertical bars, horizontal rails and concertina barbed tape topping. High...

NXP MiGLO Hearable Solutions Are Worth Listening To

NXP launched its new MiGLO family of hearable solutions today by updating their near field magnetic induction (NFMI) products under the MiGLO brand. NXP and Qualcomm must continue on separate paths while the Qualcomm acquisition of NXP is underway, however, we at TIRIAS Research can’t help but notice that the combination of Qualcomm’s CSR acquisition (closed in August 2015) with NXP’s new MiGLO hearable portfolio is huge for hearables developers. One strong validation of that capability is that NXP is already using CSR chips in its NFMI developer kit, and you can read more about our assessment of CSR’s aptX audio solution here. NXP NFMI development kit’s left ear components with NXP NFMI chip (top) and CSR8670 audio… [+] system-on-chip (SoC) (bottom) [photo: TIRIAS Research] “Hearables” are more than just simple wireless headsets (stereo speakers and a microphone sharing a Bluetooth link to a smartphone, PC or whatnot). Hearables can actively alter sound for the wearer, and are an integral part of a smartphone or app-based user experience. Also, hearables wireless sensor platforms are multi-functional. Some can measure a wearer’s heartrate, others focus on accelerometers t...

Lenovo launches Yoga Smart Tab and Smart Tab M8 tablets, Smart Display 7 at IFA

The state of Android tablet may not look all that rosy but Lenovo is bravely pushing forward. At IFA in Berlin, the company launched two new tablets. While the tablet belong to what you will say a not-so-happening category of products, Lenovo also launched a smart display with Google Assistant built-in it at the same launch event. The smart displays definitely belong to a buzzing category, with the sale of such devices on the rise. Lenovo’s smart display has a 7 inch screen. Hence its name Smart Display 7. The tablets are called Yoga Smart Tab and Smart Tab M8. The Yoga Smart Tab has a 10.1-inch screen with FullHD resolution. Built using aluminium body, it comes with a special multi-functional stand that will allow users to “tilt, stand, hold or hang their tablets”. Lenovo adds, “That means you can stand, sit, recline or lie down while using the Yoga Smart Tab, and still get a full experience at virtually any angle.” The Yoga Smart Tab is, according to Lenovo, a tablet aimed at people who want a lot of videos on their device. Along with the large 10.1-inch screen, there are JBL speakers that would likely ensure good audio experience for the Yoga Sma...

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Police will crack down on underage drinking and retailers that serve or sell alcohol to patrons under the age of 21, the legal drinking age. The crackdown will put retailers that risk breaking the law or do not check IDs on alert and, as a vital by-product, keep alcohol out of the hands of minors. Under the plan announced Monday by Gov. Andrew Cuomo, police will work with the state Liquor Authority and the state Department of Motor Vehicles to conduct underage drinking checks at establishments suspected of selling alcohol to minors, including bars, restaurants, liquor stores and grocery stores. Police will join forces with the Liquor Authority to check with local retail stores that sell beer and other forms of alcohol. There will be surprise sting operations employing underage people. More often than not, these snap inspections find stores are complying with the law. But some operations turn up establishments that flout the law. In Saratoga County, for example, a dozen establishments were cited in February following a two-day, underage alcohol sales sting. According to the state Office of Alcoholism and Substance Abuse Services, drinking alcohol before age 21 can interfere wit...

Photos: The week’s worst restaurant inspections in Jacksonville (Sept. 23) – The Florida Times-Union

Original content available for non-commercial use under a Creative Commons license, except where noted. The Florida Times-Union ~ 1 Independent Drive, Suite 200, Jacksonville, FL 32202 ~ Privacy Policy ~ Terms Of Service Egg Tray Machine, Paper Egg Tray Machine, Corrugated Carton Machine – Wanyou,https://www.wytray.com/

Potential new F1 team Panthera on their plans for 2021

21st September 2019, 15:2922nd September 2019, 12:05 | Written by Dieter Rencken and Keith Collantine Potential new F1 team Panthera is aiming to be a credible force when it arrives in the championship in 2021, its co-founder has told RaceFans. As revealed last month Panthera has already established a temporary factory in the UK, where the majority of their rivals are based. But Durand (pictured right with co-founder Michael Orts) said their long-term plan is to establish an Asian base. “Our first factory obviously would be in the UK,” he said. “But we will develop in Asia. It’s long-term, we want to develop in Asia. “We will talk about our plan when we know exactly more, but obviously for starting we need to start where the business is, where we can recruit.” The team has begun discussions with manufacturers about an engine supply. They have also been in talks with potential title sponsors, which could lead to a change from the current planned name, Panthera Team Asia F1. The team’s plans will be heavily influenced by the decisions F1 takes regarding its planned overhaul of the regulations for 2021. “[Unless] they come out with something completely crazy, so far from what we se...

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